The Science of Trenbolone

Discussion in 'Anabolic Steroids' started by plank, Jan 13, 2015.

  1. plank

    plank Member

    Sep 16, 2010
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    As we all know, trenbolone is almost mythical in its abilities as an anabolic. We have just a wealth of empirical evidence on the boards from many top level bodybuilders with ample experience on the compound. Even so, you still see a lot of contradictory recommendations and experiences.

    My goal in this thread is to break down the available scientific literature on this compound so that folks have a single resource. As with anything, you are always going to want to combine scientific literature with real-word, "in the trenches", experiences when forming your conclusions on whether trenbolone is right for you.

    Unfortunately, as we are all aware, trenbolone studies on humans don't exist so we must make due with what we have. With that said, I do think that some conclusions can be made and myths laid to rest...

    So, let's get started...
     
  2. plank

    plank Member

    Sep 16, 2010
    76
    21
    Does it Aromatize?

    http://www.ncbi.nlm.nih.gov/pubmed/782871 - Neumann F (1976)

    Trenbolone exerts a variety of anti-estrogenic effects, perhaps through hypothalamic feedback inhibition of the production of testosterone (a substrate necessary for endogenous 17β-E2/Estradiol biosynthesis). The compound is not estrogenic and seemingly not or only weakly progestationally active...

    http://europepmc.org/abstract/MED/16726369 - Renaville R et al (1988)
    http://www.ncbi.nlm.nih.gov/pubmed/6358174 - Fabry J et al (1983)

    The mechanism(s) through which trenbolone alters estrogenic activity remain to be elucidated but may be related to the inhibition of endogenous androgen synthesis (presumably through pituitary or hypothalamic feedback inhibition) and/or altered expression or activity of the aromatase enzyme. To summarize, trenbolone is not a substrate for the aromatase enzyme, but may exert both anti- and pro-estrogenic effects, with the bulk being anti-estrogenic.

    Trenbolone - How Good is it for Building Mass?

    The ability for trenbolone to promote growth is very well known. There are many studies that exist which have shown total body and skeletal muscle mass growth in various animals (bovine, rodents, etc). Here are some of the more famous studies for those wanting to review before we move along.

    K.Y. Chung, B.J. Johnson - Application of cellular mechanisms to growth and development of food producing animals -J Anim Sci, 86 (2008), pp. E226 E235

    R.J. Heitzman - The effectiveness of anabolic agents in increasing rate of growth in farm animals, report on experiments in cattle - Environ Qual Saf Suppl (1976), pp. 89-98

    P.J. Buttery, B.G. Vernon, J.T. Pearson - Anabolic agents some thoughts on their mode of action - Proc Nutr Soc, 37 (1978), pp. 311?315

    Now, we can break most of the studies out into two distinct categories...first, those studies which administered trenbolone alone and those which administered trenbolone alongside 17β-E2 (estradiol). Here is where it gets interesting...there is a lot of support for the theory that growth is superior when trenbolone is administered with estradiol than when it is administered without. This suggests that estradiol enhances the anabolic effects of trenbolone. Although the exact means by which estrogenic substances increase lean mass growth is not entirely understood, most believe it involves an indirect action on the pituitary that causes a release of bovine GH and a direct action on skeletal muscle receptors (Preston, 1987). Whereas androgens seem to exert only a direct growth effect on receptor sites in muscle tissues...

    http://www.ncbi.nlm.nih.gov/pubmed/9331864 - Henricks DM (1997) - Serum concentrations of trenbolone-17 beta and estradiol-17 beta and performance of heifers treated with trenbolone acetate, melengestrol acetate, or estradiol-17 beta.

    http://www.ncbi.nlm.nih.gov/pubmed/8617656 - Herschler et al (1995) - Production responses to various doses and ratios of estradiol benzoate and trenbolone acetate implants in steers and heifers.

    http://www.ncbi.nlm.nih.gov/pubmed/685099 - Galbraith and Watson (1978) - Performance, blood and carcase characteristics of finishing steers treated with trenbolone acetate and hexoestrol.

    http://www.ncbi.nlm.nih.gov/pubmed/573255 - Heitzman RJ (1979) - Effects of implanting prepuberal dairy heifers with anabolic steroids on hormonal status, puberty and parturition.

    http://www.ncbi.nlm.nih.gov/pubmed/9159272 - Foutz CP et al (1997) - Anabolic implant effects on steer performance, carcass traits, subprimal yields, and longissimus muscle properties.

    http://www.ncbi.nlm.nih.gov/pubmed/17431051 - Schneider et al (2007) - Effects of heifer finishing implants on beef carcass traits and longissimus tenderness.

    http://www.ncbi.nlm.nih.gov/pubmed/1526900 - Bartel et al (1992) - Trenbolone acetate/estradiol combinations in feedlot steers: dose-response and implant carrier effects.

    http://www.ncbi.nlm.nih.gov/pubmed/782875 - Heitzman's report (1976) on the anabolic growth experiments on livestock

    So, a valid argument could be made that the popular "low test / moderate mast / high tren" stack you see preached here often is not ideal for anyone whose primary goal is gaining lean mass.

    We'll talk more about trenbolone, estradiol, and fat loss in a later post but please note that most all of these studies show that marbling scores went down when trenbolone and estradiol were administered together. This further debunks the "theory" that fat loss is greater when using trenbolone in a low estrogen state.

    Please feel free to review the studies; I'll post a chart from one of them which reflects the pattern that develops in all of them though (lower marbling when trenbolone and estradiol are high).

    Trenblone's Effects on Muscle

    [​IMG]

    So, how exactly does trenbolone cause muscle growth? Although the mechanisms it uses are not entirely clear, most researchers speculate that trenbolone exerts direct anabolic effects via AR activation and associated nuclear translocation/transcription (not unlike other androgens). This is depicted in the diagram above and talked about further by Wilson, Lambright et al (2002). And, before we get too far, we must remember that trenbolone actually has been demonstrated to reduce muscle protein synthesis rates. This is far often misunderstood. The reason growth is demonstrated during use is because it reduces muscle protein degradation rates to a greater extent. Food for thought by those who preach its virtues as the anchor for bulking stacks.

    Anyway, where things get really cool though is that some literature suggests trenbolone may actually induce anabolic effects associated with either alterations to endogenous growth factors or the responsiveness of receptors in skeletal muscle to these aforementioned growth factors and IGF-1. This makes me speculate how much of a synergy may exist by using trenbolone alongside exogenous growth hormone?

    Where I feel trenbolone shines is during a diet. The reason for this is that it is fantastic, as mentioned before, in preserving lean mass via its effects on the reduction of endogenous glucocorticoid activity. It also may have the ability to suppress amino acid breakdown within the liver. I like to recommend small amounts of trenbolone as part of any extended dieting phase for these reasons.

    http://www.ncbi.nlm.nih.gov/pubmed/12441365 - Wilson, Lambright et al (2002) - In vitro and in vivo effects of 17beta-trenbolone: a feedlot effluent contaminant.

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1154211/ - Ballard and Francis (1983) - Effects of anabolic agents on protein breakdown in L6 myoblasts.

    http://www.ncbi.nlm.nih.gov/pubmed/2707149 - Thompson SH et all (1989) - Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulin-like growth factor I.


    Trenbolone's Effects on Adipose Tissue

    As a general rule, all androgens will produce secondary effects on lipolysis by binding with androgen receptors located in adipose tissue. Essentially, the stronger the androgen, the higher affinity it will have with binding to these receptors. This bind will stimulate the mobilization of fatty acids, and ultimately the oxidation of them assuming that a caloric deficit is adhered to. Trenbolone by itself, and alongside estrogen, has been shown to reduce subcutaneous fat, intramuscular fat, and decrease muscle marbling (another measurement of intramuscular fat content).

    Although, the binding of trenbolone to androgen receptors in adipose tissue is fairly straight forward, the precise mechanism(s) through which it reduces body fat remain to be determined. Many speculate it may involve a direct stimulation of lipolysis, as demonstrated by an increased expression of enzymes involved in lipolysis in the liver, including enoyl-coA-hydratase (EnoylCoA) and acyl-coA-dehydrogenase.

    In various rat studies, there has been an inability for males to gain body weight following trenbolone administration. The speculation here is that this may result from a reduction in total body fat mass or perhaps intramuscular fat content, similar to what has been observed in other species.

    K. Blouin, A. Veilleux, V. Luu-The, A. Tchernof - Androgen metabolism in adipose tissue: recent advances Mol Cell Endocrinol, 301 (2009), pp. 97?103

    R.C. Herschler, A.W. Olmsted, A.J. Edwards, R.L. Hale, T. Montgomery, R.L. Preston, et al. - Production responses to various doses and ratios of estradiol benzoate and trenbolone acetate implants in steers and heifers J Anim Sci, 73 (1995), pp. 2873?2881

    B.A. Reiling, D.D. Johnson - Effects of implant regimens (trenbolone acetate-estradiol administered alone or in combination with zeranol) and vitamin D3 on fresh beef color and quality J Anim Sci, 81 (2003), pp. 135?142

    J.A. Samber, J.D. Tatum, M.I. Wray, W.T. Nichols, J.B. Morgan, G.C. Smith - Implant program effects on performance and carcass quality of steer calves finished for 212 days J Anim Sci, 74 (1996), pp. 1470?1476

    M. Reiter, V.M. Walf, A. Christians, M.W. Pfaffl, H.H. Meyer -Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers Anal Chim Acta, 586 (2007), pp. 73?81

    Some other neat things that have been seen during rodent studies is trenbolone's somewhat unique abilities to decrease retroperitoneal (behind abdominal cavity), perirenal (kidney), and other fat depots. I have been trying to get my hands on these studies however they are unpublished, as far as I know...

    Time and time again, the lipolytic effects of trenbolone (enanthate) have been superior to testosterone (enanthate) in comparable exogenous doses. Although I would urge folks not to think of trenbolone as a "fat burner", it can definitely assist one's goals when combined with a proper diet and lifestyle.

    Summary and Recommendations

    As with most things in life, there is not a single answer to the question "how to best use trenbolone". What follows are going to be my recommendations based upon personal experience combined with the literature available on the compound.

    BULKING

    Trenbolone is a very potent size growing compound. The mechanisms of growth have been explained thoroughly so I won't repeat myself here.

    I've used trenbolone in various capacities during bulking phases and have found that my body responds best when trenbolone is not the primary growth "anchor" compound in my stack. As I said earlier, I'm fully aware that others like to run high amounts of trenbolone during bulks however this has not been the optimal way for me to obtain size, nor many others I've coached or been in contact with.

    Where trenbolone shines, traditionally, is when it is an accessory compound and used on concert with a true growth compound such as testosterone or nandrolone. The exact ratios vary, however I very much like to aim for a 2:1 ratio so that the trenbolone can exert its effects without overpowering any other compounds in the stack. Two sample growth stacks are as follows:

    150mg/week - Testosterone
    400mg/week - Trenbolone
    800mg/week - Nandrolone

    400mg/week - Trenbolone
    400mg/week - Masteron
    800mg/week - Testosterone

    CUTTING/RECOMP

    I feel this is really where trenbolone shines due to its affinity for reducing endogenous glucocorticoid activity. This means that you can be somewhat aggressive with intake deficits with little concern for proteolysis (specifically from skeletal muscle stores). In most stack configurations, I like to use AAS simply to preserve lean mass so my doses are going to be lower than is generally recommended. If you are using a less severe deficit to recomp then doses can remain higher. I'll list a couple examples of each below.

    150mg/week - Testosterone
    300mg/week - Masteron
    350mg/week - Trenbolone

    400mg/week - Testosterone
    400mg/week - Masteron
    750mg/week - Trenbolone

    As with any sample stacks, there are a lot of considerations to be made. If you are using a compound, such as trenbolone, for the first time you are going to want to start low and gradually work your way up. Furthermore, it may be in your best interest to start with the short acting (acetate) ester so you can quickly remove if from the system if adverse effects become evident.

    It should be noted that the Internet is legendary with potential sides of trenbolone and I would urge most folks to take these with a grain of salt. Far too often I feel we can become self fulfilling prophecies (i.e. you read about something, believe it will happen, and then it does). In my experience, trenbolone (although strong) is very low risk as far as producing unwanted effects.
     
  3. Titan

    Titan VIP Member

    Dec 28, 2010
    304
    84
    Tren does do what its expectations claim. But for me it shuts my nuts down hard...I try to stay away form it.
    Great post plank...
     
  4. PillarofBalance

    PillarofBalance Strength Pimp Staff Member

    Feb 27, 2011
    16,851
    4,489
    How much value can we really put into data gathered from use in cows?
     
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  5. rawdeal

    rawdeal TID Board Of Directors

    Nov 29, 2013
    1,514
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    Always wondered about reports of diminished cardio ability while on Tren. By what mechanism is it having this effect, doing something to RBC's or hemoglobin, or what? Foolishly, perhaps, cardio has never been my main priority, but I do wonder what the Tren is doing in there to cause this commonly reported side?
     
  6. Bullmuscle7

    Bullmuscle7 MuscleHead

    Jun 11, 2014
    8,848
    2,455
    Thank you for this post brother!!!
     
  7. Titan

    Titan VIP Member

    Dec 28, 2010
    304
    84
    Tren was designed to bulk up cattle before they are slaughtered.
    And many dont know but if there is a huge mass casualty, cows blood can be used for humans ( when processed properly not used directly like using human plasma). So with that little known fact; tren does have effects in humans similar to Cows.
    Reference:
    U.S. Approves Test of Blood Substitute From Cattle - NYTimes.com
     
    Last edited: Mar 7, 2015
  8. Fury

    Fury MuscleHead

    Jun 6, 2012
    1,667
    130
    Tren is one hell of a drug but abused you will pay the piper
     
  9. tommyguns2

    tommyguns2 Senior Moderators Staff Member

    Dec 25, 2010
    4,205
    1,532
    Nice post Plank. Lots of good info there. It'll take a good bit of time to go through all those links, but looking forward to it.
     
  10. Bro Bundy

    Bro Bundy MuscleHead

    Nov 1, 2012
    1,763
    332
    i love tren but the recovery was hardcore..took me months to feel normal again
     
  11. Aikido

    Aikido New Member

    Jan 26, 2014
    1
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    Thanks for the info. I have not cycled in 10 years and just grabbed 4 bottles of tren a. I was told to run this alone and
    I would see great results. everything I have read over the last 3 days ( and I've read for hours) says it will shut you
    down hard. I will grab test next week which would mean I would be doing 100mg eod for 10-12 day before I start test.
    should I stop the tren until I get test? your thoughts on this please.
     
  12. AddingPins

    AddingPins New Member

    Jan 23, 2017
    4
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    OP, kindly update your post to credit the author of this article Chest Rockwell. This is unauthorized use of his material.
     

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