Some light reading...
http://link.springer.com/article/10.1007/s00421-009-1289-x
Increased p70s6k phosphorylation during intake of a protein–carbohydrate drink following resistance exercise in the fasted state
Abstract
The present study aimed at comparing the responses of myogenic regulatory factors and signaling pathways involved in muscle protein synthesis after a resistance training session performed in either the fasted or fed state. According to a randomized crossover study design, six young male subjects participated in two experimental sessions separated by 3 weeks. In each session, they performed a standardized resistance training. After the sessions, they received during a 4-h recovery period 6 ml/kg b.w. h of a solution containing carbohydrates (50 g/l), protein hydrolysate (33 g/l), and leucine (16.6 g/l). On one occasion, the resistance exercise session was performed after the intake of a carbohydrate-rich breakfast (B), whereas in the other session they remained fasted (F). Needle biopsies from m. vastus lateralis were obtained before (Rest), and 1 h (+1h) and 4 h (+4h) after exercise. Myogenin, MRF4, and MyoD1 mRNA contents were determined by RT-PCR. Phosphorylation of PKB (protein kinase B), GSK3, p70s6k (p70 ribosomal S6 kinase), eIF2B, eEF2 (eukaryotic elongation factor 2), ERK1/2, and p38 was measured via western blotting. Compared with F, the pre-exercise phosphorylation states of PKB and p70s6k were higher in B, whereas those of eIF2B and eEF2 were lower. During recovery, the phosphorylation state of p70s6k was lower in B than in F (p = 0.02). There were no differences in basal mRNA contents between B and F. However, compared with F at +1h, MyoD1 and MRF4 mRNA contents were lower in B (p < 0.05). Our results indicate that prior fasting may stimulate the intramyocellular anabolic response to ingestion of a carbohydrate/protein/leucine mixture following a heavy resistance training session.
http://ajpendo.physiology.org/content/287/1/E1.full
Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise
Abstract
The aim of the study was to investigate the effect of resistance exercise alone or in combination with oral intake of branched-chain amino acids (BCAA) on phosphorylation of the 70-kDa S6 protein kinase (p70S6k) and mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK1/2), and p38 MAPK in skeletal muscle. Seven male subjects performed one session of quadriceps muscle resistance training (4 × 10 repetitions at 80% of one repetition maximum) on two occasions. In a randomized order, double-blind, crossover test, subjects ingested a solution of BCAA or placebo during and after exercise. Ingestion of BCAA increased plasma concentrations of isoleucine, leucine, and valine during exercise and throughout recovery after exercise (2 h postexercise), whereas no change was noted after the placebo trial. Resistance exercise led to a robust increase in p70S6k phosphorylation at Ser424 and/or Thr421, which persisted 1 and 2 h after exercise. BCAA ingestion further enhanced p70S6k phosphorylation 3.5-fold during recovery. p70S6k phosphorylation at Thr389 was unaltered directly after resistance exercise. However, during recovery, Thr389 phosphorylation was profoundly increased, but only during the BCAA trial. Furthermore, phosphorylation of the ribosomal protein S6 was also increased in the recovery period only during the BCAA trial. Exercise led to a marked increase in ERK1/2 and p38 MAPK phosphorylation, which was completely suppressed upon recovery and unaltered by BCAA. In conclusion, BCAA, ingested during and after resistance exercise, mediate signal transduction through p70S6k in skeletal muscle.
Mark Burkham's take on the subject...
Myth: Fasted training sucks. You'll lose muscle and have no strength.
Truth
A large body of research on sports performance during Ramadan concludes that aerobic activities, such as 60 minutes of running, has a small yet significant negative impact on performance. A very large confounder here is dehydration, as Ramadan fasting involves fluid restriction. That said, anaerobic performance, such as weight training, is much less impacted.
However, more relevant and telling studies, which don't involve fluid restriction, show that strength and lower intensity endurance training is unaffected - even after 3.5 days of fasting. New research on fasted training supports this. If you read my review of that study, you'll see that the only parameter the fed group did better on was improvements in V02max, which is likely explained by the fact that the carbs allowed them to train at a higher intensity. However, note the other interesting results obtained in the fasted group. Also note that a review I did of another fasted endurance training study showed no negative effect of fasting on endurance or VO2max (quite the contary in fact). This can be explained by the lower intensity.
In conclusion, training in the fasted state does not affect your performance during weight training, which is what most people reading this are interested in. However, training in a completely fasted state is still not something I recommend for optimal progress. Research is quite clear on the benefits of pre-workout and post-workout protein intake for maximizing protein synthesis. For this reason, I suggest supplementing with 10 g BCAA prior to fasted training.