Finally worked up the nerve to do it. Don't know what I was so worried about it was quite simply. Mix, heat, filter. The stuff looks beautiful. I made it at 200mg per ml and just shot 1ml in my delt. Now wait to see if I get an infection :food-snacking:
Finally worked up the nerve to do it. Don't know what I was so worried about it was quite simply. Mix, heat, filter. The stuff looks beautiful. I made it at 200mg per ml and just shot 1ml in my delt. Now wait to see if I get an infection :food-snacking:
I did run in to one little problem. The brake bleed pump was tested before connecting and it worked. I connected it to the nalgene filter and couldn't get suction. Just didn't have it on tight enough. You really have to crank that thing on but I was afraid of cracking the filter...
Only question I have left though is how to you avoid contamination going from the media bottle to a syringe. You have to uncap it. They aren't for sale so I don't need even amounts in the vials (used 30ml vials) but the vials I used were pre sterilized and sealed. I suppose with uncapped vials you can just pour out the vacuum spout into the vial. How do you guys do that part?
Room needs to be really clean obviously and the air should be filtered in the room... You can also filter twice, use a filter cup then load up a 60ml syringe and put a filter on it then fill vials 6 at a time or in ur case 2...
I wouldn't worry so much about the filter breaking as much as the cup, so glass is better.
Finally worked up the nerve to do it. Don't know what I was so worried about it was quite simply. Mix, heat, filter. The stuff looks beautiful. I made it at 200mg per ml and just shot 1ml in my delt. Now wait to see if I get an infection :food-snacking:
I did run in to one little problem. The brake bleed pump was tested before connecting and it worked. I connected it to the nalgene filter and couldn't get suction. Just didn't have it on tight enough. You really have to crank that thing on but I was afraid of cracking the filter...
Only question I have left though is how to you avoid contamination going from the media bottle to a syringe. You have to uncap it. They aren't for sale so I don't need even amounts in the vials (used 30ml vials) but the vials I used were pre sterilized and sealed. I suppose with uncapped vials you can just pour out the vacuum spout into the vial. How do you guys do that part?
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