Oct 19, 2010 I think everyone knows getting off is a function of the testes, also that the testes and testosterone production are directly related. If the testes are busy making more semen every time you jack off will that entail testosterone production? basically, are testosterone levels higher when one partakes in masterbation frequently or lower? I personally would think masterbation would have an effect on the testes ability to produce testosterone if it is busy making semen. but then again I have no clue.