A
argentiniansteak
Member
- Apr 28, 2012
- 18
- 0
I have collated the following methods for keeping things clean / sterilisation. Can comebody pick the best method from this list:
For All Stuff
-------------
1. The number one thing you should be thinking of is practicing
isolation of sterilized surfaces and items. That is, if a piece is sterile, be sure that it doesn't touch anything that is non-sterile. If your hands touch something that you haven't sterilized, swap gloves. Think of it as a tattoo artist, a piercer, or your doc would. Cross-contamination can wreck the final product.
2. Work in an area or on a tray that you have wiped down with
antiseptic solution before use and make sure you are working in
an area with no air flow OR under a laminar flow hood(If you have
access or know how to make one). If you are using pre-sterilized
and sealed vials the airflow issue isnt as important.
Easiest Method
--------------
Wash with a non-residue soap
Rinse with ISO-alcohol
Bake at 225 for 15 or more minutes and use as soon as it cools enough
Dont use anything you haven't cleaned right before you use it
Easy Method
-----------
Put everything in the dish washer
Rinse with Benzyl alcohol before use
Easy Method
-----------
Wash and heat dry everything before bagging.
Clean everything directly before and after use.
Alternative Method
------------------
Using hot water and the Glass Magic or something like that will remove the particulates from inside of the vials
Use the dishwasher liquid that leaves glass spotless - have that in solution with hot water - dunk the vials in them. Rinse them with regular hot water multiple times, then the final solution rinse should be distilled water.
Then heat sterilize.
A proper sanitation rinse is probably better than rubbing alcohol as far as residue is concerned. Either way, the dry heat sterilization will work fine for this regardless of the alcohol being applied before baking or not.
Thresh Method
-------------
Alcohol bath. Then right in a convention oven @ 250 degrees for 20 min. Turn oven off, remove with rubber gloves from oven AS you need them. Your good to go.
Can microwave them for a few min if you want to.
darkrid3r Method
----------------
I do mine like this:
Wash all parts in anti-bacterial soap and hot water (I use a baby bottle nipple cleaner with stainless steel brush tip to clean the inside of all bottles), rinse with hot water, from here they go into 50% alch solution, from here onto a pan with aluminum foil top down.
Place in oven at 350 for 30 minutes.
Rubber stoppers get done this way:
Wash in anti-bacterial soap, rinsed in hot water, sit in alch solution, placed face UP(this means the part of the stopper that goes inside the vial is face up)
Build brew, take vials out of oven, let cool to the touch while you finish brew.
Move vials from pan to counter, vilter into vial. stopper as soon as the vial is at the apropriate level.
Cap and your done.
There is no need to bake a second time, as long as your workspace is clean (i like to use anti-bacterial wipes on everything all the time) you should be fine.
I have made and ran 50 weeks of product this way with not a single issue ever.
2% ba, 18%bb
I also do all my lab ware the very same way.
Media bottles can be done this way, but its much more difficult to clean the inside, IMO best to use single use bottles unless your equiped to clean the inside of hte bent neck ones.
I have never seen a residue from the alch that I use, Pickup at drug store, its standard rubbing alch that you can get at any drugstore, pharmacy, costco, food store etc.
BA only prevents the bacteria from growing INSIDE the vial, if its already in there your too late. Once this bacteria gets into the body it now has a nice warm moist enviroment to grow and cause potential issues. BA is an inhibitor not a killer.
Method 1
--------
1. Put everything in a dishwasher or put everything in the autoclave
2. Rinse everything with benzyl alcohol before use.
Method 2
--------
1. Clean the vials
2. Wash with soap and distilled water
3. Rinse with distilled water
4. Rinse a second time with isopropyl
5. Dry and heat your vials
6. Place upside down on a baking sheet that has been wiped with an antiseptic
7. Preheat oven to 350 degrees fahrenheit
8. Place in the oven and let set for 30 mins.
9. Allow vials to cool to room temperature and then repeat the procees.
10. Soak stoppers in hydrogen peroxide for 10 mins.
All other items you use that should be sterilised come factoru sterilised e.g; suringes, needles, filters
Method 3
--------
1. Wash with a non-residue soap
2. Rinse with Isopropyl alcohol
3. Bake for 15 or more minutes
4. Use as soon as its cool enough.
5. Dont use anything you havent cleaned right before using it.
Important Info
--------------
Calculations
Untitled
EO Based Stuff - USE PTFE Filters (Whatman PTFE Polycaps?) - USE 3% BA Total
1. Prefilter EO with 1% BA with a filter then throw away.
2. Filter hormone with BA/BB and any oil seperately.
3. Add sterile EO
4. Use 3% BA Total
5. Let brew site 1 week before injecting
Steps for Preparation
---------------------
1. Prepare solution to be filtered
2. If solution has been heated make sure temperature does not excees 122 Degrees Fahrenheit (50 Degress Celcius). High temperature may melt the filter or damage the filter membrane.
For All Stuff
-------------
1. The number one thing you should be thinking of is practicing
isolation of sterilized surfaces and items. That is, if a piece is sterile, be sure that it doesn't touch anything that is non-sterile. If your hands touch something that you haven't sterilized, swap gloves. Think of it as a tattoo artist, a piercer, or your doc would. Cross-contamination can wreck the final product.
2. Work in an area or on a tray that you have wiped down with
antiseptic solution before use and make sure you are working in
an area with no air flow OR under a laminar flow hood(If you have
access or know how to make one). If you are using pre-sterilized
and sealed vials the airflow issue isnt as important.
Easiest Method
--------------
Wash with a non-residue soap
Rinse with ISO-alcohol
Bake at 225 for 15 or more minutes and use as soon as it cools enough
Dont use anything you haven't cleaned right before you use it
Easy Method
-----------
Put everything in the dish washer
Rinse with Benzyl alcohol before use
Easy Method
-----------
Wash and heat dry everything before bagging.
Clean everything directly before and after use.
Alternative Method
------------------
Using hot water and the Glass Magic or something like that will remove the particulates from inside of the vials
Use the dishwasher liquid that leaves glass spotless - have that in solution with hot water - dunk the vials in them. Rinse them with regular hot water multiple times, then the final solution rinse should be distilled water.
Then heat sterilize.
A proper sanitation rinse is probably better than rubbing alcohol as far as residue is concerned. Either way, the dry heat sterilization will work fine for this regardless of the alcohol being applied before baking or not.
Thresh Method
-------------
Alcohol bath. Then right in a convention oven @ 250 degrees for 20 min. Turn oven off, remove with rubber gloves from oven AS you need them. Your good to go.
Can microwave them for a few min if you want to.
darkrid3r Method
----------------
I do mine like this:
Wash all parts in anti-bacterial soap and hot water (I use a baby bottle nipple cleaner with stainless steel brush tip to clean the inside of all bottles), rinse with hot water, from here they go into 50% alch solution, from here onto a pan with aluminum foil top down.
Place in oven at 350 for 30 minutes.
Rubber stoppers get done this way:
Wash in anti-bacterial soap, rinsed in hot water, sit in alch solution, placed face UP(this means the part of the stopper that goes inside the vial is face up)
Build brew, take vials out of oven, let cool to the touch while you finish brew.
Move vials from pan to counter, vilter into vial. stopper as soon as the vial is at the apropriate level.
Cap and your done.
There is no need to bake a second time, as long as your workspace is clean (i like to use anti-bacterial wipes on everything all the time) you should be fine.
I have made and ran 50 weeks of product this way with not a single issue ever.
2% ba, 18%bb
I also do all my lab ware the very same way.
Media bottles can be done this way, but its much more difficult to clean the inside, IMO best to use single use bottles unless your equiped to clean the inside of hte bent neck ones.
I have never seen a residue from the alch that I use, Pickup at drug store, its standard rubbing alch that you can get at any drugstore, pharmacy, costco, food store etc.
BA only prevents the bacteria from growing INSIDE the vial, if its already in there your too late. Once this bacteria gets into the body it now has a nice warm moist enviroment to grow and cause potential issues. BA is an inhibitor not a killer.
Method 1
--------
1. Put everything in a dishwasher or put everything in the autoclave
2. Rinse everything with benzyl alcohol before use.
Method 2
--------
1. Clean the vials
2. Wash with soap and distilled water
3. Rinse with distilled water
4. Rinse a second time with isopropyl
5. Dry and heat your vials
6. Place upside down on a baking sheet that has been wiped with an antiseptic
7. Preheat oven to 350 degrees fahrenheit
8. Place in the oven and let set for 30 mins.
9. Allow vials to cool to room temperature and then repeat the procees.
10. Soak stoppers in hydrogen peroxide for 10 mins.
All other items you use that should be sterilised come factoru sterilised e.g; suringes, needles, filters
Method 3
--------
1. Wash with a non-residue soap
2. Rinse with Isopropyl alcohol
3. Bake for 15 or more minutes
4. Use as soon as its cool enough.
5. Dont use anything you havent cleaned right before using it.
Important Info
--------------
Calculations
Untitled
EO Based Stuff - USE PTFE Filters (Whatman PTFE Polycaps?) - USE 3% BA Total
1. Prefilter EO with 1% BA with a filter then throw away.
2. Filter hormone with BA/BB and any oil seperately.
3. Add sterile EO
4. Use 3% BA Total
5. Let brew site 1 week before injecting
Steps for Preparation
---------------------
1. Prepare solution to be filtered
2. If solution has been heated make sure temperature does not excees 122 Degrees Fahrenheit (50 Degress Celcius). High temperature may melt the filter or damage the filter membrane.
