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Hello all,
I need help again.
I have few Hybex glass media bottles.
How I can sterilysed them to reuse them with security for my homebrews?
Very hot water and soap then rices with very hot water after that you can do 2 of 2 things or both and that is use bleach then alcohol at 91% or one of the other, if you were to do just one I would do alcohol.
If you have a dishwasher you can buy sterilizing soap and wash and dry it in there.
Just wash with dish soap and rinse with distilled water. Then bake in the oven at 170+ C for at least 60 minutes (that is the microbiology guideline for dry heat sterilization).
Wash the media bottles with dish soap and distilled water. Get a baby bottle cleaner, looks like a pipe cleaner.
Once you scrubbed it with soap and distilled water, rinse it off with distilled water. (Never let tap water touch your glass, that means dedicate a sponge to glassware cleaning)
Then I'd rinse with 99% iso alcohol. ONLY USE 99%, it acts like a solvent.
Fill the bottle with some iso alcohol and use that baby bottle cleaner. Rinse with distilled water.
Visually inspect each bottle after you rinsed with distilled water.
If you want to sterilize; heat the bottles in the oven to 400 degrees for 60min, let cool to about 200 degrees(turn off heat for ~60min), then heat again to 350 degrees for 60min. I was once told bacteria can adapt to extreme heat, and the fluctuation of extreme heat will for sure kill off any bacteria.
Wow, another quality post there SFGiants. Don't listen to this guy, you'll get an abscess.Originally Posted by SFGiants
Found this on a search here on TID.
Sterilizing vials can be done a number of ways. The key to UG vial sterilization is two fold. #1. Keeping all particulate contaminants out. #2. Removal of all endotoxins.
Easy way:
1. Vials are washed in Dionized or Distilled water to remove particulate matter. Use a bottle brush dedicated for cleaning vials or bottles only. Rinse with DI or Distilled water. Rinse taking care when removing vials from rinse to keep them upside down so you don't get particulates in the vials. (Keep air flow to zero)
2. Vials are placed in stainless steel pans and sterilized in dry heat for 5 hours @ 250oC. This accomplishes the sterilization and depyrogenation process.
3. Closures (stoppers) are steam sterilized @ 121oC for 30 minutes, then dried for 2 to 8 hours @105oF.
Added Dry Heat Sterilization times below
170C (340F) 60 minutes
160C (320F) 120 minutes
150C (300F) 150 minutes
140C (285F) 180 minutes
121C (250F) overnight
Moderately difficult way:
1. Vials are washed with DI or Distilled water to remove particulate matter, rinsed with DI or Distilled water. Take care when removing vials from rinse to keep them upside down so you don't get particulates in the vials. (Keep air flow to zero)
2. Pressure steam sterilize at 18-21 psi for 35 minutes.
3. Vials are placed upside down in stainless steel pan and dried at 285 for 1-2 hours.
The key is DI water or Distilled water. If you use tap water you will leave mineral deposits in your vials when you dry them.
For media bottles I might add that you want to cool down your media bottles very slowly otherwise you will have condensation accumulate in your bottles.
2nd attacked me on both making an ass of yourself because both posts stand true you just don't like me for some reason and are starting shit, ask yourself how long that's going to last and what will come out of it because it's useless wasted energy.
You made a stupid comment about upright rows and got corrected by another member and now this, I learned this method on the this board by very well known and respected person on here.
Abscess my ass!
I'm done with this and I'm done with you, ignore button in seconds.
Get a life bro and stop starting shit up on the board!
Last edited by SFGiants; 07-12-2012 at 09:25 PM.
Silly easy and it works.
anti-bacterial dish soap if you wanta be a techy bastad~ ! lol
actualy a hydrogen-peroxide wash 20% if you can get it followed by distilled water.
IF you only have Hydrogen-peroxide 5% than soak and wash for at least 5 minutes of contact time. (1 minute with 20%)
several ways to score with this damn thing.
For you guys using the alcohol 91% or above ,soak and wash for at least 1 minute contact time.
Pay attention to your contact times with the solvents, with the heating you all will be fine.
I havn't seen any bad advice posted in this thread,
Lets discuss it with respect to others or GTFO of the thread!
Last edited by SHINE; 07-09-2012 at 01:48 AM.
You guys can laugh at this but these things get freaking HOT.
Medela Quick Clean Micro-Steam Bags - 5ct - Medela - Babies "R" Us
Product Description
Medelas unique and powerful Quick Clean Micro-Steam Bags are designed and tested for disinfecting breastpump parts and feeding accessories. Proven effective to eliminate harmful bacteria and germs by 99.9%. Complete steam-cleaning in just 3 minutes. Faster than boiling and more effective than dishwashing. Just add water and items to clean, and heat in the microwave. Each bag will hold two breastshield and valve assemblies or two, 5oz collection bottles and caps. 5 reusable bags per box (up to 20 uses per bag = 100 uses). Easy to store and transport.
Nice find, I believe in steam cleaning never thought of it for media bottles but you have me thinking.
Bleach makes the house have a crap smell and the alcohol makes it smell like Gin factor.
Some people use a dishwasher with the drying cycle as that gets damn hot also but I'm thinking the steam is much better as it's way more scalding.
Just picked some up today, almost went with the bottle cleaner thing but rather have something disposable.
Thanks bro, these are amazing!
Them Medela quick clean bags... U use distilled water in them ?
I wanted to touch on something here that I have been reading up on.
Medical / surgical tool are sterilized with only 91% alcohol, read up on it.
Some hospitals may do it different but I have read the this is the norm.
They let it soak a few minutes and that's it.
Bleach and Alcohol is not necessary to do both just one or the other but I would always use extreme hot water and soap 1st.
A lot of top dogs in the game do this it's how I 1st learned about it.
Also like Lizard was pointing out and I do this also, steam IMO is the best to sterilize.
Wouldn't methanol be more effective ? The key is unless you have an autoclave with sealing bags (helps if you have friends in tatooing) these ways work well.
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